Thermo Fisher Scientific CD4 Monoclonal Antibody (S3.5), Qdot 655
인간 CD4 항원을 인식하는 Mouse IgG2a 단클론 항체로, Qdot 655 형광체로 결합되어 높은 감도와 밝은 형광을 제공합니다. UV 또는 405nm 광원에서 최적의 여기, 유세포분석에 적합하며 다중 색 분석에 유리합니다. 연구용으로만 사용됩니다.
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Thermo Fisher Scientific CD4 Monoclonal Antibody (S3.5), Qdot 655
Applications
- Flow Cytometry (Flow): Assay-dependent
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Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | S3.5 |
| Immunogen | Human CD4 |
| Conjugate | Qdot™ 655 |
| Excitation / Emission Max | 300 / 654 nm |
| Form | Liquid |
| Purification | Purified |
| Storage Buffer | 0.05M borate, pH 8.3, with 1M betaine |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11180600 |
Additional Formats
- Unconjugated
- Alexa Fluor 405, 488, 700
- APC, APC-Alexa Fluor 750, APC-Cyanine5.5
- FITC, Pacific Blue, Pacific Orange
- PE, PE-Alexa Fluor 610, 700
- PE-Cyanine5.5, PE-Cyanine7, PE-Texas Red
- PerCP, Qdot 605, 705, 800
- TRI-COLOR
- Request custom conjugation
Product Specific Information
Qdot™ Antibody conjugates provide bright fluorescence suitable for detecting low-abundance extracellular proteins. They are similar in size to R-phycoerythrin (R-PE) and compatible with existing fluorophore conjugates. Best excited by UV or violet light, Qdot conjugates have narrow emission profiles and long Stokes shifts, enabling efficient multicolor assays using a 405 nm laser.
Staining:
Use standard buffers such as PBS with 1% BSA. Analyze cells within 18 hours of staining. Dilute only what is needed for one day. Compatible with reagents such as Cal-Lyse™ Lysing Solution and FIX & PERM™ reagents. Some BD FACS™ Lysing Solution batches may interfere with fluorescence.
Instrument Setup:
Optimal excitation at 405 nm (also excitable by 488 nm). Recommended filter: 655/20 nm. Ensure cytometer filters capture emission maxima for accurate analysis.
Storage:
Store at 2–8°C in the dark. Do not freeze. Expires six months from receipt unless otherwise indicated. Photostable and resistant to light degradation. Contains cadmium and selenium; dispose per local regulations.
Target Information
CD4 antigen participates in MHC class II recognition and acts as a co-receptor for HIV. Expressed mainly in T helper lymphocytes, also found in monocytes, macrophages, dendritic cells, thymus, lymph nodes, tonsils, spleen, and some non-lymphoid tissues. CD4 aids in T-cell activation via association with TCR and Lck kinase and may mediate neuronal damage in immune-related CNS diseases.
Multiple alternatively spliced transcripts identified (RefSeq, July 2017).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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