Thermo Fisher Scientific p21 Monoclonal Antibody (GT8611)
인간 p21 단백질을 인식하는 마우스 단클론 항체로, Western blot, ICC/IF, Flow Cytometry에 사용 가능. Protein G 정제, 1 mg/mL 농도의 액상 형태. p53 관련 세포주기 조절 연구에 적합하며, 단기 4°C, 장기 -20°C 보관 권장.
- 판매단위
- pk
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Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
| Flow Cytometry (Flow) | Assay-dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GT8611 |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human p21 Cip1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2787111 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Cat (84%), Sheep (82%), Rhesus Monkey (98%), Bovine (83%).
- Positive Control: HeLa, HCT116 (30 µM Cisplatin treatment for 24 hr).
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Protein p21 is a tumor suppressor protein and an inhibitor of Cyclin-dependent kinases (CDK), transcriptionally activated by p53. It plays a key role in the cellular response to genotoxic stress and acts as a major transcriptional target of p53.
In the nucleus, p21 binds and inhibits CDK1 and CDK2, blocking cell cycle progression from G1 to S phase or G2 to M phase after DNA damage, allowing DNA repair.
In the cytoplasm, p21 exhibits anti-apoptotic activity by inhibiting caspase 3 and apoptotic kinases ASK1 and JNK. p21 thus has dual roles in carcinogenesis, acting both as a tumor suppressor and as an oncogene depending on context.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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