Thermo Fisher Scientific p21 Monoclonal Antibody (GT1032)
Thermo Fisher Scientific의 p21 Monoclonal Antibody (GT1032)는 인간 및 생쥐 시료에 반응하는 고순도 단일클론 항체로, Western blot, IHC, ICC/IF 등 다양한 응용에 적합합니다. Recombinant p21 단백질을 면역원으로 사용하며, 단백질 A로 정제된 액상 형태로 제공됩니다.
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Applications 및 Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | - |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | 1 publication |
| Flow Cytometry (Flow) | Assay-dependent | - |
| Immunoprecipitation (IP) | 1:100–1:500 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GT1032 |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human p21 Cip1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.8 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2787110 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Cat (84%), Sheep (82%), Rhesus Monkey (98%), Bovine (83%).
- Positive Control: HeLa, HCT116 (30 µM Cisplatin treatment for 24 hr), HCT116 (30 µM Cisplatin treatment for 48 hr).
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Protein p21 is a tumor suppressor protein that inhibits Cyclin-dependent kinases (CDK) and is transcriptionally activated by p53.
p21 plays a key role in cellular response to genotoxic stress and is a major transcriptional target of p53.
In the nucleus, p21 binds and inhibits Cdk1 and Cdk2, blocking transition from G1 to S phase or G2 to mitosis after DNA damage, enabling DNA repair.
In the cytoplasm, p21 has an anti-apoptotic effect, binding to and inhibiting caspase 3, ASK1, and JNK.
p21 exhibits dual roles in carcinogenesis — acting both as a tumor suppressor and as an oncogene depending on context.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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