Thermo Fisher Scientific Factor X/Xa Monoclonal Antibody (GT268)

Applications

Western Blot (WB)

• Tested Dilution: 1:5,000–1:20,000

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:100–1:1,000

Product Specifications

Product Specific Information

• Keep as a concentrated solution.
• Predicted reactivity: Rhesus Monkey (93%).
• Positive Control: human F10-transfected 293T cells.
• Centrifuge briefly prior to opening the vial.

Target Information

Factor X undergoes multiple processing steps before its preproprotein is converted to a mature two-chain form by the excision of the tripeptide RKR. Two chains of the factor are held together by one or more disulfide bonds; the light chain contains two EGF-like domains, while the heavy chain contains the catalytic domain, homologous to other hemostatic serine proteases.
The mature factor is activated by cleavage of the activation peptide by factor IXa (intrinsic pathway) or factor VIIa (extrinsic pathway). The activated factor then converts prothrombin to thrombin in the presence of factor Va, Ca²⁺, and phospholipid during blood clotting.
Mutations of this gene result in factor X deficiency, a hemorrhagic condition of variable severity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.