Thermo Fisher Scientific Rat anti-Mouse IgG2a Secondary Antibody, PerCP-eFluor 710, eBioscience

Applications

Tested Dilution

Flow Cytometry (Flow)

0.25 µg/test

Product Specifications

Host/Isotype

Rat / IgG1, kappa

Class

Monoclonal

Type

Secondary Antibody

Clone

m2a-15F8

Conjugate

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

View additional formats

• [APC ](/antibody/secondary/product-name/Mouse IgG2a/conjugates/APC/host/Rat/)
• [Biotin ](/antibody/secondary/product-name/Mouse IgG2a/conjugates/Biotin/host/Rat/)
• [eFluor 570](/antibody/secondary/product-name/Mouse IgG2a/conjugates/eFluor 570/host/Rat/)
• [FITC](/antibody/secondary/product-name/Mouse IgG2a/conjugates/FITC/host/Rat/)
• [HRP](/antibody/secondary/product-name/Mouse IgG2a/conjugates/HRP/host/Rat/)
• [PE ](/antibody/secondary/product-name/Mouse IgG2a/conjugates/PE/host/Rat/)
• [PE-Cyanine7](/antibody/secondary/product-name/Mouse IgG2a/conjugates/PE-Cyanine7/host/Rat/)
• [Super Bright 436](/antibody/secondary/product-name/Mouse IgG2a/conjugates/Super Bright 436/host/Rat/)
• [Super Bright 600](/antibody/secondary/product-name/Mouse IgG2a/conjugates/Super Bright 600/host/Rat/)
• [Super Bright 780](/antibody/secondary/product-name/Mouse IgG2a/conjugates/Super Bright 780/host/Rat/)
• Request custom conjugation

Excitation/Emission Max

482/708 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Wet ice

RRID

AB_2573756

Target

IgG2a

Antibody Form

Whole Antibody

Product Specific Information

Description: The monoclonal antibody m2a-15F8 recognizes mouse IgG2a antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other mouse isotypes nor does it crossreact to rat IgG2a or any rat isotype antibodies.

Applications Reported: This m2a-15F8 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This m2a-15F8 antibody has been tested by flow cytometric analysis of cells stained with a mouse IgG2a primary antibody. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.