Thermo Fisher Scientific Phospho-CD247 (CD3 zeta) (Tyr142) Monoclonal Antibody (3ZBR4S), PE, eBioscience

Thermo Fisher Scientific Phospho-CD247 (CD3 zeta) (Tyr142) Monoclonal Antibody (3ZBR4S), PE, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.25 µg)/test

Product Specifications

Additional Formats

• Alexa Fluor 488
• APC
• eFluor 450
• Custom conjugation available

Product Specific Information

This 3ZBR4S monoclonal antibody recognizes human and mouse CD247 (CD3z) phosphorylated on Tyrosine 142.
CD3 is a multi-protein complex expressed on thymocytes and T cells, essential for T cell receptor (TCR) signaling.
The CD3 zeta chain initiates the signaling cascade following TCR engagement and contains three ITAM motifs phosphorylated by Src kinases such as Lck.
Phosphorylated ITAMs recruit ZAP-70, amplifying TCR signaling.
Loss or mutation of CD3 zeta impairs T cell development and function.
Phosphorylation occurs early in the TCR signaling cascade, downstream of Lck and upstream of ZAP-70, LAT, and SLP-76.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested by flow cytometric analysis of stimulated normal human peripheral blood cells
Recommended Usage: 5 µL (0.25 µg) per test (100 µL final volume; 10⁵–10⁸ cells/test)
Excitation: 488–561 nm; Emission: 578 nm; Laser: Blue, Green, Yellow-Green Laser

Target Information

The CD3z complex transduces antigen-recognition signals into T cells and regulates TCR surface expression.
CD3 subunits (gamma, delta, epsilon, zeta) are immunoglobulin superfamily members encoded on chromosome 11.
They contain cytoplasmic tails with tyrosine-based motifs mediating signal transduction.
CD3 antigen is present on most T lymphocytes and thymocytes but absent on B or NK cells.
Associated diseases include Immunodeficiency 25 and T-B+ Severe Combined Immunodeficiency due to CD3delta/CD3epsilon/CD3zeta defects.

For Research Use Only. Not for use in diagnostic procedures.

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