Thermo Fisher Scientific CD80 (B7-1) Chimeric Recombinant Rabbit Monoclonal Antibody (RM80)
Thermo Fisher Scientific의 CD80 (B7-1) Chimeric Recombinant Rabbit Monoclonal Antibody (RM80)은 마우스 CD80 단백질을 특이적으로 인식하는 항체로, WB, IHC, ICC/IF, Flow 등 다양한 분석에 사용 가능하며, HEK293 발현 시스템 기반의 고순도 단백질 A 정제 항체입니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (IHC) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| Flow Cytometry (Flow) | Assay-dependent |
| Functional Assay (Functional) | Assay-dependent |
| Inhibition Assays (IA) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RM80 |
| Immunogen | This antibody was raised by immunising Sprague Dawley rats with a mouse B cell line, BCL1, and fusing immune splenocytes with P3U1 myeloma cells. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3074575 |
Product Specific Information
Specificity: This antibody is specific for murine CD80, a ~55 kDa member of the Ig superfamily.
Target Information
CD80 (B7-1) and CD86 (B7-2) are ligands of T cell costimulatory molecule CD28 and inhibitory receptor CTLA-4 (CD152). Both are expressed on professional antigen-presenting cells and are essential for T cell activation. CD80 is rapidly induced on activated B cells, EBV-transformed B cell lines, Burkitt’s lymphoma cell lines, follicular B lymphoma cells, T cells, and monocytes. The B-lymphocyte activation antigen B7-1 provides regulatory signals for T lymphocytes via binding to CD28 and CTLA4. Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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