Thermo Fisher Scientific Myosin Skeletal Muscle Monoclonal Antibody (MY32)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:25-1:50

-

Immunocytochemistry (ICC/IF)

Assay-dependent

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Rabbit, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

MY32

Immunogen

Rabbit muscle myosin. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Myosin Skeletal Muscle,uniProtId:Q28641-1,ncbiNodeId:9986,antigenRange:1-1938,antigenLength:1938,antigenImageFileName:MA1-90701_Myosin_Skeletal_Muscle_Q28641-1_House_mouse.svg,antigenImageFileNamePDP:MA1-90701_Myosin_Skeletal_Muscle_Q28641-1_House_mouse_PDP.jpeg,sortOrder:4}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_1955676

Product Specific Information

This antibody recognizes heavy chain human skeletal myosin (fast-twitch type II isomyosin). It does not stain cardiac or smooth muscle myosin.

For antigen retrieval, it is recommended to use a citrate buffer, pH 6.0, at room temperature for 30 min.

Store as a concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Target Information

Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosin heavy chains are encoded by a multigene family. In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and nonmuscle cells. These isoforms show expression that is spatially and temporally regulated during development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.