Thermo Fisher Scientific Myosin Skeletal Muscle Monoclonal Antibody (MY32)
골격근 미오신 중 사슬을 인식하는 단클론 항체로, 빠른 수축형(type II) 미오신에 특이적입니다. Western blot, IHC, ICC/IF 등 다양한 응용에 적합하며, 심근 및 평활근 미오신에는 반응하지 않습니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:25–1:50 | - |
| Immunocytochemistry (ICC/IF) | Assay-dependent | 1 publication |
| Miscellaneous PubMed (Misc) | - | 1 publication |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Rabbit, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MY32 |
| Immunogen | Rabbit muscle myosin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Ascites |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
| RRID | AB_1955676 |
Product Specific Information
This antibody recognizes heavy chain human skeletal myosin (fast-twitch type II isomyosin). It does not stain cardiac or smooth muscle myosin.
For antigen retrieval, use citrate buffer (pH 6.0) at room temperature for 30 minutes.
Store as a concentrated solution. Centrifuge briefly prior to opening vial and keep at 4°C.
Target Information
Myosin is a major contractile protein that converts chemical energy into mechanical energy through ATP hydrolysis. It is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains.
Myosin heavy chains are encoded by a multigene family. In mammals, at least ten different MYH isoforms have been described from striated, smooth, and nonmuscle cells. These isoforms show spatial and temporal regulation during development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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