Thermo Fisher Scientific Chicken anti-Goat IgG (H+L) Secondary Antibody, Biotin
Biotin 결합 Chicken anti-Goat IgG (H+L) 2차 항체로, ELISA·WB·ICC·Flow 등 다양한 면역실험에 사용 가능. Goat IgG에 높은 특이성과 감도를 제공하며, 친화 크로마토그래피로 정제됨. 연구용으로만 사용.
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Thermo Fisher Scientific Chicken anti-Goat IgG (H+L) Secondary Antibody, Biotin
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000 |
| Immunocytochemistry (ICC/IF) | 1:500–1:20,000 |
| Flow Cytometry (Flow) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Goat |
| Host / Isotype | Chicken / IgY |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobulins Heavy and Light chains |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; -20°C long term (avoid freeze/thaw cycles) |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2534641 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Available Formats
- [AP conjugate]
- [FITC conjugate]
- [HRP conjugate]
- [TRITC conjugate]
- [Request custom conjugation]
Product Specific Information
Each antibody lot is tested for sensitivity using ELISA and for specificity by immunoelectrophoresis (IEP).
Target Information
Anti-Goat secondary antibodies are affinity-purified reagents with well-characterized specificity for goat immunoglobulins. They are useful for detection, sorting, or purification of target proteins.
Secondary antibodies enhance versatility and sensitivity by enabling multiple detection systems (HRP, AP, fluorescence) and signal amplification.
Typically, secondary antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species and can be further purified or modified (immunoaffinity chromatography, antibody fragmentation, label conjugation) for high specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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