Thermo Fisher Scientific DNMT2 Monoclonal Antibody (1E12)
DNMT2 단백질을 인식하는 Mouse monoclonal antibody (Clone 1E12). Western blot, ICC/IF, ELISA에 사용 가능. Human 시료 반응성. PBS buffer, no preservative. -20°C 보관, 동결/해동 반복 피할 것.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–5 µg/mL |
| Immunocytochemistry (ICC/IF) | 40 µg/mL |
| ELISA | 10 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, lambda |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1E12 |
| Immunogen | DNMT2 (AAH47733, 1 a.a. ~ 391 a.a.) full-length recombinant protein with GST tag (GST tag MW: 26 kDa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Protein sequence:
MEPLRVLELY SGVGGMHHAL RESCIPAQVV AAIDVNTVAN EVYKYNFHHT QLLAKTIEGI TLEEFDRLSF DMILMSPPCQ PFTRIGRQGD MTDSRTNSFL YILDILPRLQ KLPKYILLEN VKGFEVSSTR DLLIQTIENC GFQYQEFLLS PTSLGIPNSR LRYFLIAKLQ SEPLPFQAPG QVLMEFPKIE SVHPQKYAMD VEEKIQEKNV EPNISFDGSI QCSGKDAILF KLETAEEIHR KNQQDSDLSV KMLKDFLEDD TDVNQYLLPP KSLLRYALLL DIVQPTCRRS VCFTKGYGSY IEGTGSVLQT AEDVQVENIY KSLTNLSQEE QITKLLILKL RYFTPKEIAN LLGFPPEFGF PEKITVKQRY RLLGNSLNVH VVAKLIKILY E
Target Information
CpG methylation is an epigenetic modification essential for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have shown that DNA methylation is required for mammalian development. This gene encodes a protein similar to DNA methyltransferases but lacks methyltransferase activity. The protein strongly binds DNA, suggesting it may mark specific genomic sequences. Alternative splicing results in multiple transcript variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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