Thermo Fisher Scientific HLA-ABC Monoclonal Antibody (W6/32), NovaFluor Blue 510, eBioscience

Thermo Fisher Scientific HLA-ABC Monoclonal Antibody (W6/32), NovaFluor Blue 510, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.6 µg)/test

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Product Specifications

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Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• Biotin
• Brilliant UV 395
• Brilliant UV 615
• Brilliant UV 737
• Brilliant Violet 650
• Brilliant Violet 786
• FITC
• Functional Grade
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 590
• NovaFluor Yellow 660
• NovaFluor Yellow 755
• PE
• PE-Cyanine5
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

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Product Specific Information

The W6/32 monoclonal antibody reacts with the human major histocompatibility complex (MHC) class I, HLA-A, B, C.
MHC class I antigens associated with beta 2-microglobulin are expressed by all human nucleated cells and play a central role in cell-mediated immune response and tumor surveillance.
W6/32 mAb recognizes a non-polymorphic epitope shared among HLA-A, B, and C loci and immunoprecipitates both 43 kDa and 11–12 kDa chains. Crossreactivity is seen in baboon, rhesus, and cynomolgus monkey.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells.
Use at 5 µL (0.6 µg) per test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for NovaFluor dyes.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
  Add 5 µL per sample before combining antibody cocktails with cells.
  For single-color controls, use 5 µL per 100 µL of cell sample (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology, employing nucleic acid dye structures for engineered fluorescent signatures with minimized spillover and spread.

Excitation: 496 nm
Emission: 511 nm
Laser: 488 nm (Blue)

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Target Information

HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and associated with beta2 microglobulin.
They present foreign determinants to cytotoxic T cells, critical for antiviral and antitumor defense.
Human HLA-class I antigens include HLA-A, HLA-B, and HLA-C molecules.
HLA and MHC antibodies are essential in Immunopeptidomics, enabling identification and characterization of neoantigens via LC-MS/MS.

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For Research Use Only. Not for use in diagnostic procedures or resale without express authorization.

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