Thermo Fisher Scientific RTN1 Monoclonal Antibody (RNL-2)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:25-1:200

-

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

-

Immunocytochemistry (ICC/IF)

Assay-dependent

-

Flow Cytometry (Flow)

1:25-1:200

-

Product Specifications

Species Reactivity

Human, Non-human primate, Rabbit

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

RNL-2

Immunogen

Small cell lung cancer cell line NCI-H82. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:RTN1,uniProtId:Q16799-1,ncbiNodeId:9606,antigenRange:1-776,antigenLength:776,antigenImageFileName:MA1-06813_RTN1_Q16799-1_House_mouse.svg,antigenImageFileNamePDP:MA1-06813_RTN1_Q16799-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_561123

Product Specific Information

MA1-06813 detects reticulon-1A(NSP-A) and reticulon-1B(NSP-B) in human, rabbit, and non-human primate cells.

MA1-06813 has sucessfully been used in Western blotting, immunocytochemistry, FACS and immunohistochemistry (paraffin) procedures. By Western blot it detects a ~135 kDa band representing reticulon-1A, and a ~45 kDa band representing reticulon-1B.

The MA1-06813 immunogen is small cell lung cancer cell line NCI-H82.

Target Information

Reticulon-1 was formerly designated NSP for Neuroendocrine-Specific-Protein, because it is specifically expressed in neural and neuroendocrine tissues. The NSP-gene has been mapped by fluorescence in situ hybridization to human chromosome 14q21-q22. The NSP-gene encodes three overlapping proteins, i.e. reticulon-1A (NSP-A), reticulon-1B (NSP-B), and reticulon-1C (NSP-C). These proteins were found to be anchored to membranes of the endoplasmic reticulum through their common carboxy-terminal regions. Reticulon-1A is a protein with a molecular weight of approximately 135 kDa, which occurs in various isoforms presumably depending on the degree of phosphorylation of serine residues. In lung cancer diagnosis Reticulon-1A appeared to be a reliable marker for the detection of neuroendocrine differentiation, since most of the small cell lung carcinoma (SCLC) and carcinoid tumors showed expression of Reticulon-1A. Reticulon-1B is a phosphoprotein with a MW of 45 kDa and is restricted to the lung cancer cell line NCI-H82. Reticulon-1B is so far not found in human tissues. Reticulon-1C is a protein with a MW of 23 kDa which is not phosphorylated and is found with Reticulon-1A in SCLC (cell lines) and not in non-SCLC (cell cultures).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.