Thermo Fisher Scientific CD146 Monoclonal Antibody (P1H12), NovaFluor Blue 610-70S, eBioscience

Applications

• Flow Cytometry (Flow)
  • Tested Dilution: 5 µL (0.2 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• eFluor 450
• eFluor 660
• FITC
• NovaFluor Blue 660-120S
• NovaFluor Yellow 730
• PE
• PE-Cyanine7
• Request custom conjugation available

Product Specific Information

Description:
Monoclonal antibody P1H12 recognizes CD146 (also known as MUC18, s-endo, Endo-CAM, Mel-CAM), a member of the Ig superfamily. CD146 is expressed on endothelial cells, bone marrow fibroblasts, some tumors (notably melanoma), mesenchymal stromal cells, and endometrial stromal cells. Expression on circulating blood cells is limited to a subset of T cells, not endothelial cells. The protein is heavily glycosylated, with over 50% carbohydrate mass. P1H12 crossreacts with mouse, rabbit, and canine, but not rat.

Each product includes 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested on normal human peripheral blood cells and HUVEC.
Use 5 µL (0.02 µg) per test in 100 µL final volume. Cell number: 10⁵–10⁸ cells/test.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains with NovaFluor dyes.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) must be used for optimal staining.
  • Add 5 µL per sample before mixing with cells.
  • For single-color controls: 5 µL per 100 µL of sample containing 10³–10⁸ cells.

Technology:
NovaFluor conjugates are based on Phiton™ technology, utilizing nucleic acid dye structures engineered for optimized fluorescence with minimal spectral spillover.

Excitation / Emission / Laser:
509 nm / 614 nm / 488 nm (Blue)

Target Information

CD146 (MCAM):
An integral membrane glycoprotein of the immunoglobulin superfamily expressed at endothelial cell junctions and various cell types. Functions as a calcium-independent cell adhesion molecule and may act in signal transduction via Fyn kinase activation, influencing actin cytoskeleton assembly. Plays a role in endothelial cohesion, tumor metastasis (melanoma), and neural crest cell adhesion during development.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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