Thermo Fisher Scientific Mouse IgG1 Isotype Control, Biotin

Applications

Tested Dilution

Flow Cytometry (Flow)

Assay-dependent

Control (Ctrl)

Assay-Dependent

Product Specifications

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Isotype Control

Immunogen

Human T lymphocytes

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.1 mg/mL

Purification

Protein G

Storage buffer

PBS with 1% BSA

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2550616

Product Specific Information

PA5-33199 has been tested and found to be negative on the following rat tissues - peripheral blood leucocytes, thymocytes, splenocytes and macrophages. PA5-33199 recognizes the human CD45RA antigen, and therefore human leucocytes may be used as a positive control for this product. This antibody is not suitable for use as a negative control on human tissues.

For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Target Information

Mouse IgG1 is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG1 is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation. Mouse IgG1 is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry. It is also used in therapeutic applications, such as immunotherapy and vaccine development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.