Thermo Fisher Scientific RACGAP1 Polyclonal Antibody
RACGAP1 단백질을 인식하는 Thermo Fisher Scientific의 goat polyclonal antibody. Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용에 적합. 고순도 IgG fraction으로 제조되어 높은 특이성과 재현성을 제공. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.3–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1 µg/mL |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| Flow Cytometry (Flow) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide with sequence GRQGNFFASPMLK, from C terminus of the protein sequence according to NP_037409 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | IgG fraction |
| Storage Buffer | TBS, pH 7.3, with 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2176161 |
Product Specific Information
- Recommended positive controls: The peptide used to generate this antibody is available for purchase (GTX22270-PEP).
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
RAC, a small GTPase in the ras family, regulates the formation of membrane ruffles, lamellipodia, and filopodia. It is involved in cytoskeletal actin organization and transformation. The p21 activated kinases (PAKs) are direct targets of active Rac and Cdc42, which can induce the assembly of polarized cytoskeletal structures when expressed in fibroblasts. RAC1 and RAC2 are also essential components of NADPH oxidase, the enzyme responsible for generating free radicals.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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