Thermo Fisher Scientific WIBG Monoclonal Antibody (OTI11C5), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:150

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of about 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

WIBG is a key regulator of the exon junction complex (EJC), a multiprotein complex that associates immediately upstream of exon-exon junctions on mRNAs. It serves as a positional landmark for the intron-exon structure and directs post-transcriptional processes such as mRNA export, nonsense-mediated decay (NMD), and translation. WIBG acts as an EJC disassembly factor, allowing translation-dependent removal and recycling of EJC from spliced mRNAs. Its association with the 40S ribosomal subunit restricts activity to translated mRNAs, preventing premature disassembly. WIBG interferes with NMD and enhances translation of spliced mRNAs by antagonizing EJC functions. RNA-binding has been observed (PubMed:14968132), though its relevance in vivo remains unclear.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.