Thermo Fisher Scientific PREB Polyclonal Antibody
Human, Mouse, Rat 반응성을 가진 Rabbit Polyclonal PREB 항체로 Western blot 및 IHC(P) 실험에 적합. Antigen affinity chromatography로 정제된 0.1 mg/mL 액상 항체. PBS/glycerol buffer에 보존되며 장기 보관 시 -20°C 권장.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:20–1:50 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human PREB. Recombinant protein control fragment (Product #RP-90969). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2645927 |
Product Specific Information
Immunogen sequence:
REAHGIVVTD VAFLPEKGRG PELLGSHETA LFSVAVDSRC QLHLLPSRRS VP
Highest antigen sequence identity:
- Mouse: 90%
- Rat: 90%
Target Information
Prolactin (PRL) expression in the pituitary is limited to specific cells. Pit-1 is a pituitary-specific transcription factor that plays an important role in PRL expression, both in mature organism and during development. The PRL promoter contains numerous Pit-1 binding sites implicated in basal and kinase-mediated gene expression.
A novel protein, termed PREB (Prolactin Regulatory Element Binding Protein), regulates PRL gene expression through the 1P site, though it lacks a discernable DNA-binding motif. Studies indicate that PREB is encoded by a single-copy gene in both mice and humans and exhibits nuclear accumulation in pituitary cells. Evidence suggests PREB acts as a transcription factor assisting PRL expression alone or with Pit-1.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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