Thermo Fisher Scientific Gata-3 Monoclonal Antibody (TWAJ), PE-Cyanine5, eBioscience
Gata-3 단백질을 인식하는 TWAJ 클론 단일클론 항체로, PE-Cyanine5로 표지되어 있습니다. 인간, 마우스, 돼지, 원숭이에 반응하며, 세포내 염색 후 유세포분석에 적합합니다. Foxp3/Transcription Factor Staining Buffer Set 사용 권장. 빛에 민감하므로 어둡게 보관하십시오.
- 카탈로그번호
- 15-9966-42
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Gata-3 Monoclonal Antibody (TWAJ), PE-Cyanine5, eBioscience
Thermo Fisher Scientific Gata-3 Monoclonal Antibody (TWAJ), PE-Cyanine5, eBioscience™
Applications and Tested Dilution
- Application: Flow Cytometry (Intracellular Staining)
- Tested Dilution: 5 µL (0.125 µg)/test
- Publications: View 10 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Pig, Rhesus monkey |
| Published Species | Not Applicable |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), PE-Cyanine5, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | TWAJ |
| Conjugate | PE-Cyanine5 |
| Excitation / Emission Max | 568 / 666 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2811756 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 700
- Brilliant UV 395
- Brilliant UV 563
- Brilliant UV 661
- Brilliant Violet 421
- Brilliant Violet 480
- Brilliant Violet 605
- Brilliant Violet 711
- Brilliant Violet 786
- eFluor 450
- eFluor 660
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
The monoclonal antibody TWAJ recognizes mouse and human Gata-3, a transcription factor critical for thymic development and Th2 differentiation. Gata-3 is expressed in the central nervous system, skin, mammary glands, and kidney during embryonic development, and is essential for T cell commitment and survival. Expression is mainly found on CD4 single positive thymocytes.
Alternative splice variants (50 and 45 kDa) are recognized by this antibody.
Staining requires the Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00).
Cross-reactivity in rhesus monkeys has been reported.
- Applications Reported: Intracellular staining followed by flow cytometric analysis
- Applications Tested: Pre-diluted and validated using Foxp3/Transcription Factor Staining Buffer Set
- Recommended Use: 5 µL (0.125 µg) per 100 µL cell sample (10⁵–10⁸ cells/test)
Light Sensitivity: This tandem dye is sensitive to photo-induced oxidation. Protect from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-57) for up to 3 days at 4°C in the dark.
Excitation: 488–561 nm
Emission: 667 nm
Compatible Lasers: Blue, Green, Yellow-Green
Target Information
GATA3 is a zinc finger transcription factor that binds enhancer elements of T-cell receptor (TCR) genes (alpha, beta, gamma, delta). It regulates TCR gene expression and is abundantly expressed in T-lymphocytes but not in B cells or macrophages. The human GATA3 gene spans 17 kb with 6 exons, encoding two conserved zinc finger domains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.