Thermo Fisher Scientific Gata-3 Monoclonal Antibody (TWAJ), PE-Cyanine5, eBioscience

Thermo Fisher Scientific Gata-3 Monoclonal Antibody (TWAJ), PE-Cyanine5, eBioscience™

Applications and Tested Dilution

• Application: Flow Cytometry (Intracellular Staining)
• Tested Dilution: 5 µL (0.125 µg)/test
• Publications: View 10 publications

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 700
• Brilliant UV 395
• Brilliant UV 563
• Brilliant UV 661
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 605
• Brilliant Violet 711
• Brilliant Violet 786
• eFluor 450
• eFluor 660
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The monoclonal antibody TWAJ recognizes mouse and human Gata-3, a transcription factor critical for thymic development and Th2 differentiation. Gata-3 is expressed in the central nervous system, skin, mammary glands, and kidney during embryonic development, and is essential for T cell commitment and survival. Expression is mainly found on CD4 single positive thymocytes.

Alternative splice variants (50 and 45 kDa) are recognized by this antibody.
Staining requires the Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00).
Cross-reactivity in rhesus monkeys has been reported.

• Applications Reported: Intracellular staining followed by flow cytometric analysis
• Applications Tested: Pre-diluted and validated using Foxp3/Transcription Factor Staining Buffer Set
• Recommended Use: 5 µL (0.125 µg) per 100 µL cell sample (10⁵–10⁸ cells/test)

Light Sensitivity: This tandem dye is sensitive to photo-induced oxidation. Protect from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-57) for up to 3 days at 4°C in the dark.

Excitation: 488–561 nm
Emission: 667 nm
Compatible Lasers: Blue, Green, Yellow-Green

Target Information

GATA3 is a zinc finger transcription factor that binds enhancer elements of T-cell receptor (TCR) genes (alpha, beta, gamma, delta). It regulates TCR gene expression and is abundantly expressed in T-lymphocytes but not in B cells or macrophages. The human GATA3 gene spans 17 kb with 6 exons, encoding two conserved zinc finger domains.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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