Thermo Fisher Scientific FnCpf1 Monoclonal Antibody (9H6), GenCRISPR

Applications and Tested Dilutions

Product Specifications

Product Specific Information

Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/mL.

Cpf1-containing CRISPR-Cas loci of Francisella tularensis subsp. novicida U112 encode functional defense systems capable of mediating plasmid interference in bacterial cells guided by the CRISPR spacers.

Unlike Cas9 systems, Cpf1-containing CRISPR systems have three features:

1. Cpf1-associated CRISPR arrays are processed into mature crRNAs without the requirement of an additional trans-activating crRNA (tracrRNA).
2. Cpf1-crRNA complexes efficiently cleave target DNA preceded by a short T-rich protospacer adjacent motif (PAM), in contrast to the G-rich PAM required for Cas9 systems.
3. Cpf1 introduces a staggered DNA double-stranded break with a 4–5 nt 5′ overhang.

Target Information

Clustered Regularly Interspaced Short Palindromic Repeats from Prevotella and Francisella 1 (CRISPR/Cpf1) is a DNA-editing technology analogous to the CRISPR/Cas9 system.
Cpf1 is an RNA-guided endonuclease of a class II CRISPR/Cas system, found in Prevotella and Francisella bacteria.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.