Thermo Fisher Scientific beta-2 Adaptin Polyclonal Antibody
Western blot 및 Immunocytochemistry에 적합한 beta-2 Adaptin 폴리클로날 항체. 사람, 마우스, 랫드, 소 시료에서 반응. 항원 친화 크로마토그래피로 정제된 1 mg/mL 액상 항체. PBS/BSA 완충액에 보관하며 -20°C에서 냉동 보관.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 2 µg/mL |
| Immunocytochemistry (ICC/IF) | 2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to residues I(588)HRKHLP IHHGSTDAGDSP(606) of human beta Adaptin 2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2056349 |
Product Specific Information
- PA1-1066 detects beta Adaptin 2 from human, mouse, rat, and bovine samples.
- Successfully used in Western blot procedures.
- Immunogen: synthetic peptide corresponding to residues I(588)HRKHLP IHHGSTDAGDSP(606) of human beta Adaptin 2.
- Can be used with blocking peptide PEP-293.
Target Information
Clathrin-mediated endocytosis is the pathway by which many receptors for nutrients and hormones are internalized for recycling or down-regulation. During formation of clathrin-coated membranes, clathrin co-assembles with heterotetrameric molecules known as assembly polypeptides (APs) or adaptors, forming a protein coat between the clathrin lattice and the membrane.
There are two characterized adaptors, AP1 and AP2.
- AP1: Associated with clathrin-coated vesicles at the trans-Golgi network.
- AP2: Associated with endocytic clathrin-coated vesicles at the plasma membrane; interacts with Shc and EGF receptor.
AP2 is composed of four subunits: two ~100 kDa gene products (alpha and beta adaptin) and two smaller subunits (~50 kDa and ~17 kDa). There are two alpha-adaptin genes, alpha A and alpha C, each with tissue-specific expression patterns.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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