Thermo Fisher Scientific CH25H Polyclonal Antibody
CH25H 단백질을 인식하는 토끼 폴리클로날 항체로, Western blot, IHC, ELISA 등에 사용 가능. 인간 단백질에 반응하며, 친화 크로마토그래피로 정제됨. 액상 형태로 제공되며 -20°C에서 보관. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:100 |
| ELISA | 1:10,000–1:50,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Anti-CH25H antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-terminal region of human CH25H conjugated to Keyhole Limpet Hemocyanin (KLH). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.92 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02 M potassium phosphate, pH 7.2, with 0.15 M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This affinity-purified antibody is directed against human CH25H. A BLAST analysis suggests cross-reactivity with the antigen based on 100% homology with the immunizing sequence, and 92% homology to pig, 91% to rat, and 75% to mouse.
Target Information
CH25H catalyzes the formation of 25-hydroxycholesterol from cholesterol, repressing cholesterol biosynthetic enzymes. It plays a key role in cell positioning and movement in lymphoid tissues, as 25-hydroxycholesterol is an intermediate in the biosynthesis of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC), a ligand for the G protein-coupled receptor GPR183/EBI2.
It may regulate lipid metabolism by synthesizing a corepressor that blocks SREBP processing. In testis, 25-hydroxycholesterol produced by macrophages may influence Leydig cell differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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