Thermo Fisher Scientific mCherry Polyclonal Antibody
mCherry 단백질 및 그 변이체(RFP, tdTomato 등)에 특이적인 Rabbit Polyclonal 항체. Western blot, IHC, ELISA, IP 등 다양한 응용에 사용 가능. 높은 특이성과 민감도를 제공하며, -20°C에서 안정적으로 보관 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:200–1:2,000 |
| ELISA | 1:150,000–1:250,000 |
| Immunoprecipitation (IP) | 1:2,000–1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | mCherry mutant variant fusion protein of RFP corresponding to the full-length amino acid sequence (234 aa) derived from Discosoma (mushroom polyp coral) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02 M potassium phosphate, pH 7.2, with 0.15 M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Avoid repeated freeze/thaw cycles.
- Centrifuge if not clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
Reactivity:
Reacts with mCherry, RFP, and its variants (tdTomato, mBanana, mOrange, mPlum, mStrawberry).
No cross-reactivity with Human, Mouse, or Rat serum proteins.
Specificity confirmed by ELISA (<0.1% off-target signal).
Immunoelectrophoresis shows a single precipitin arc against anti-Rabbit Serum and purified mCherry.
Target Information
mCherry is a monomeric fluorescent protein used as a tracer and molecular marker in biotechnology.
It absorbs at 587 nm and emits at 610 nm, showing high photostability and rapid maturation (t₀.₅ ≈ 15 minutes), allowing visualization shortly after expression.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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