Thermo Fisher Scientific SMARCB1 Polyclonal Antibody
SMARCB1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB, IHC, ICC, ELISA, IP 등 다양한 응용에 적합. 인간, 마우스, 랫트 반응성. 고순도 Affinity Chromatography 정제 및 안정적 PBS/glycerol 저장.
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Thermo Fisher Scientific SMARCB1 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5 µg–4 µg antibody for 200 µg–400 µg extracts |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 50–150 of human SMARCB1/SNF5 (NP_003064.2) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2.49 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2914520 |
Product Specific Information
- Positive test controls: SH-SY5Y, THP-1, HepG2, HeLa, Mouse testis, Rat testis
- Subcellular localization: Nucleus
- Immunogen sequence:
LWRRLATVEE RKKIVASSHG KKTKPNTKDH GYTTLATSVT LLKASEVEEI LDGNDEKYKA VSISTEPPTY LREQKAKRNS QWVPTLPNSS HHLDAVPCSTT
Target Information
The SWI-SNF complex is involved in the activation of transcription via remodeling of nucleosome structure in an ATP-dependent manner. Brm (SNF2α) and Brg-1 (SNF2β) are ATPase subunits of the mammalian SWI-SNF complex. Brm, Brg-1, Ini1 (SNF5), BAF155 (SRG3), and BAF170 form the functional core of the complex. Addition of Ini1, BAF155, and BAF170 to Brg-1 increases remodeling activity. Other subunits play regulatory roles. hSNF2L and hSNF2H are homologs of Drosophila ISWI, present in chromatin remodeling complexes such as NURF.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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