Thermo Fisher Scientific NUCB2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000-1:2,500

-

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human NUCB2. Recombinant protein control fragment (Product #RP-88817).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2644853

Product Specific Information

Immunogen sequence: MSEVDTNKDR LVTLEEFLKA TEKKEFLEPD SWETLDQQQF FTEEELKEYE NIIALQENEL KKKADELQKQ KEELQRQHDQ LEAQKLEYHQ VIQQMEQKKL QQGIPPSGPA GELKFEPR

Highest antigen sequence identity to the following orthologs: Mouse - 86%, Rat - 86%.

Target Information

NUCB2 was initially identified as calcium-binding protein EF hand motif-containing protein that could bind with Necdin, a growth suppressor expressed primarily in postmitotic neurons. NUC2 is a secreted protein that is cleaved into three major peptide products, Nesfatin-1, Nesfatin-2, and Nesfatin-3, of which Nesfatin-1 has been found to cause the suppression of food intake in a leptin-independent manner. Other studies have suggested that NUCB2/Nesfatin-1 may also play roles in energy homeostasis and closely related neuroendocrine functions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.