Thermo Fisher Scientific M-cadherin Polyclonal Antibody
Human M-cadherin을 인식하는 sheep polyclonal antibody로, WB, ICC/IF, Flow Cytometry에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, PBS 및 5% 트레할로스 버퍼에 동결건조 상태로 제공됩니다. 근육 세포 분화 연구에 유용합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| Immunocytochemistry (ICC/IF) | 5–15 µg/mL |
| Flow Cytometry (Flow) | 2.5 µg per million cells |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Sheep / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant human M-Cadherin/Cadherin-15 (Val22–Ala606) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2608493 |
Product Specific Information
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
Cadherins are a multigene family of calcium-dependent cell adhesion molecules. They are transmembrane glycoproteins composed of an extracellular domain that mediates Ca²⁺-dependent intercellular adhesion through homophilic interactions, a transmembrane region, and a cytoplasmic domain. The extracellular domain consists of subdomains EC1–EC5. Homologies among cadherin family members are highest in the cytoplasmic domain and EC1/EC2 regions, and lower in EC5 and the transmembrane region. Binding specificity resides primarily in the N-terminal portion.
Four major cadherins have been identified in mammals: neuronal (N), epithelial (E), placental (P), and muscle (M) cadherins. M-cadherin is absent in fibroblasts but expressed at low levels in myoblasts and upregulated during myotube formation, suggesting a role in skeletal muscle cell differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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