Thermo Fisher Scientific p53 Monoclonal Antibody (DO-7), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:150

Product Specifications

Product Specific Information

• For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
• For conjugation experiments using this carrier-free antibody, perform an additional desalting step.
  Recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882.

Target Information

The tumor suppressor protein p53 is a sequence-specific transcription factor activated by cellular stress. It mediates cell cycle arrest or apoptosis in response to DNA damage or nucleotide starvation.
p53 activation leads to transcription of genes such as p21waf1, regulating G1 or G2/M arrest. Structurally, p53 includes:

• N-terminal transactivation domain
• Central DNA-binding domain
• Oligomerisation domain
• C-terminal regulatory domain

Phosphorylation at Ser15 is critical for activation and stabilization, while Ser392 phosphorylation is associated with tumor formation.
p53 functions as an anticancer agent, blocking proliferation of damaged cells, and is linked to aging, oxidative stress, and learning/memory deficits due to altered LTP.
Mutations in TP53 lead to loss of tumor suppressor activity and are found in many cancers, including Li-Fraumeni syndrome.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.