Thermo Fisher Scientific C/EBP alpha Monoclonal Antibody (15C8)

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

View 5 publications 5 publications

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

View 2 publications 2 publications

ChIP assay (ChIP)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Non-human primate

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

15C8

Immunogen

Synthetic peptide corresponding to residues M(1) E S A D F Y E V E P R P P(14) of mouse C/EBP alpha. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:C/EBP alpha,uniProtId:P53566-1,ncbiNodeId:10090,antigenRange:2-14,antigenLength:359,antigenImageFileName:MA1-825_CEBP_alpha_P53566-1_House_mouse.svg,antigenImageFileNamePDP:MA1-825_CEBP_alpha_P53566-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2077903

Product Specific Information

MA1-825 detects C/EBP alpha protein in human, mouse and rat samples.

MA1-825 has successfully been used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects a 45 kDa protein representing C/EBP alpha in transfected hu293T cells. MA1-825 is specific to the amino terminal region of C/EBP alpha.

The MA1-825 immunogen is a synthetic peptide corresponding to residues M(1) E S A D F Y E V E P R P P(14) of mouse C/EBP alpha. This peptide (Cat. # PEP-242) is available for use in neutralization and control experiments.

Target Information

The C/EBP (CCAAT/enhancer binding protein) family of transcription factors--alpha, beta, delta, and epsilon)--are widely expressed proteins found in a variety of tissues. These domains contain an evolutionarily conserved five-amino acid motif (the regulatory domain motif RDM) that conforms to the consensus sequence (I/V/L) KXEP. One of the major roles of these transcription factors is that of metabolic regulation in liver and fat cells. Studies suggest that these transcription factors act in a cascade aiding in the development of the adipose cell. Three C/EBP beta isoforms (LAP*, LAP, and LIP) arise from different translation initiation and display different function in gene regulation. From a neurological standpoint, C/EBPs have been implicated in the mediation of nerve growth factor, altering synaptic plasticity, and mediation of inflammatory response in AD patients.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.