Thermo Fisher Scientific C/EBP alpha Monoclonal Antibody (15C8)
C/EBP alpha 단백질을 검출하는 마우스 모노클로날 항체로, Western blot 및 Immunoprecipitation에 적합. 인간, 마우스, 랫트 시료에서 반응. 단백질 G 정제, PBS/BSA 완충액에 저장되며 -20°C에서 보관. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 2 µg/mL | View 5 publications |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Immunoprecipitation (IP) | Assay-dependent | View 2 publications |
| ChIP assay (ChIP) | - | View 2 publications |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Mouse, Non-human primate |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 15C8 |
| Immunogen | Synthetic peptide corresponding to residues M(1) E S A D F Y E V E P R P P(14) of mouse C/EBP alpha |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2077903 |
Product Specific Information
MA1-825 detects C/EBP alpha protein in human, mouse, and rat samples.
It has been successfully used in Western blot and immunoprecipitation procedures.
By Western blot, this antibody detects a 45 kDa protein representing C/EBP alpha in transfected hu293T cells.
MA1-825 is specific to the amino terminal region of C/EBP alpha.
The immunogen is a synthetic peptide corresponding to residues M(1) E S A D F Y E V E P R P P(14) of mouse C/EBP alpha.
This peptide (Cat. # PEP-242) is available for use in neutralization and control experiments.
Target Information
The C/EBP (CCAAT/enhancer binding protein) family of transcription factors—alpha, beta, delta, and epsilon—are widely expressed in various tissues.
These domains contain an evolutionarily conserved five-amino acid motif (the regulatory domain motif RDM) that conforms to the consensus sequence (I/V/L) KXEP.
C/EBP transcription factors play key roles in metabolic regulation in liver and fat cells and act in cascades aiding adipose cell development.
Three C/EBP beta isoforms (LAP*, LAP, and LIP) arise from different translation initiation sites and display distinct functions in gene regulation.
C/EBPs are also implicated in nerve growth factor mediation, synaptic plasticity, and inflammatory response in Alzheimer’s disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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