Thermo Fisher Scientific TPRKB Monoclonal Antibody (OTI1E9), TrueMAB
TPRKB 단백질을 인식하는 Mouse monoclonal antibody (Clone OTI1E9). Western blot과 IHC(P)에서 검증됨. 동결건조 형태로 제공되며, 재구성 후 약 1 mg/mL 농도로 사용 가능. 연구용으로 EKC/KEOPS 복합체 관련 tRNA 변형 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1E9 |
| Immunogen | Full length human recombinant protein of human TPRKB produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
TPRKB is a component of the EKC/KEOPS complex required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. The complex is likely involved in transferring the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37.
TPRKB acts as an allosteric effector regulating the t6A activity of the complex but is not required for tRNA modification.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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