Thermo Fisher Scientific C-Jun Monoclonal Antibody (UMAB49), UltraMAB
c-Jun 단백질을 인식하는 UMAB49 클론의 마우스 단일클론 항체. WB, IHC, ICC, ChIP 등 다양한 실험에 적용 가능. 고순도 친화 크로마토그래피 정제, 동결건조 형태로 제공. 연구용 전용 제품으로 안정적인 보관 및 재용해 용이.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| ChIP assay (ChIP) | Assay-dependent |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | UMAB49 |
| Immunogen | Full length human recombinant protein of human JUN produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
c-Jun is a transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'.
It promotes NR5A1 activity when phosphorylated by HIPK3, leading to increased steroidogenic gene expression upon cAMP signaling stimulation.
Also involved in KRAS-mediated transcriptional activation of USP28 in colorectal cancer cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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