Thermo Fisher Scientific CD31 (PECAM-1) Monoclonal Antibody (WM-59 (WM59)), PerCP-eFluor 710, eBioscience
인간 CD31(PECAM-1)을 인식하는 WM-59 단클론 항체로, PerCP-eFluor 710 형광체가 결합된 형광표지 항체입니다. 유세포분석(Flow Cytometry)에 최적화되어 있으며, 고순도의 Affinity Chromatography 정제 제품입니다. 4°C 암소 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.125 µg)/test | View 33 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PerCP-eFluor™ 710 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | WM-59 (WM59) |
| Conjugate | PerCP-eFluor™ 710 |
| Excitation / Emission Max | 482 / 708 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1834397 |
Additional Formats Available
- Unconjugated
- APC
- APC-eFluor 780
- Biotin
- eFluor 450
- FITC
- Functional Grade
- PE
- PE-Cyanine7
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Request custom conjugation available
Product Specific Information
The WM59 monoclonal antibody reacts with human CD31 (PECAM-1, gpIIa), a 130–140 kDa surface protein expressed by endothelial cells and at low levels on leukocytes and platelets. CD31 plays a role in cell adhesion, cell–cell and cell–matrix interactions, and signal transduction.
Applications Reported:
This antibody is validated for flow cytometric analysis.
Applications Tested:
Pre-titrated and tested for flow cytometric analysis of normal human peripheral blood cells. Recommended usage is 5 µL (0.125 µg) per test (100 µL final volume). Suitable for 10⁵–10⁸ cells/test.
Fluorochrome Information:
PerCP-eFluor® 710 emits at 710 nm and is excited by the blue laser (488 nm). Recommended filter: 710/50 BP (695/40 BP acceptable alternative). Ensure instrument compatibility.
Fixation:
Samples may be stored up to 3 days at 4°C in the dark using:
- IC Fixation Buffer (Product #00-8222-49)
- 1-step Fix/Lyse Solution (Product #00-5333-54)
Clone-specific performance after fixation should be verified empirically.
Excitation / Emission / Laser:
488 nm / 710 nm / Blue Laser
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
CD31 (PECAM-1) is an inhibitory coreceptor that regulates T and B cell signaling via ITIM motifs, providing docking sites for protein-tyrosine phosphatases upon phosphorylation. It is mainly located at endothelial cell junctions and mediates homophilic binding important for cell–cell interactions. CD31 functions in integrin-mediated adhesion, transendothelial migration, angiogenesis, apoptosis, immune regulation, and thrombosis. It is a key regulatory molecule in the vascular system.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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