Thermo Fisher Scientific EGF Monoclonal Antibody (10827)

Applications and Tested Dilution

Product Specifications

Product Specific Information

In sandwich ELISAs, no cross-reactivity or interference is observed with recombinant mouse (rm) EGF, bovine FGF acidic, bovine FGF basic, recombinant human (rh) G-CSF, rhGM-CSF, rmGM-CSF, rhLIF, human PDGF, porcine PDGF, rhTGF‑alpha, hTGF-beta 1, pTGF-beta 1, rhTGF-beta 1, porcine TGF-beta 1.2, porcine TGF-beta 2, rhTNF-alpha, or rhTNF-beta.

Reconstitute at 0.5 mg/mL in sterile PBS.

Target Information

Epidermal Growth Factor (EGF) binds to the EGF receptor (EGFR), a 170 kDa protein kinase, to initiate cellular signaling pathways. Upon activation, EGFR forms homo- or heterodimers with other family members, triggering distinct signaling cascades through recruitment of SH2-containing effector proteins.
EGF is widely expressed in kidney, cerebrum, prostate, and salivary glands, acting as a potent mitogenic factor. The phosphorylated receptor recruits adapter proteins such as GRB2, activating downstream cascades including RAS-RAF-MEK-ERK, PI3K-AKT, PLCγ-PKC, and STAT pathways.
Research suggests EGF also plays a role in NF-κB signaling activation. Mutations in the EGF gene are linked to hypomagnesemia type 4, and dysregulation is associated with certain cancers.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.