Thermo Fisher Scientific TNFR1 Monoclonal Antibody (16805)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

-

ELISA (ELISA)

0.1-0.4 µg/mL (Detection), 2-8 µg/mL (Capture)

-

Neutralization (Neu)

1-6 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

16805

Immunogen

E. coli-derived recombinant human TNF RI/TNFRSF1A Extracellular domain

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.5 mg/mL

Purification

Protein A/G

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2609265

Product Specific Information

In sandwich ELISAs, no significant cross-reactivity or interference with recombinant human (rh) TGF-alpha, hTGF-beta 1, porcine (p) TGF-beta 1, rhTGF-beta 1, pTGF-beta 1.2, pTGF-beta 2, recombinant chicken TGF-beta 3, rhTGF-beta 3, recombinant amphibian TGF-beta 5, rhTNF-beta, or rhTNF RII is observed.

Reconstitute at 0.5 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

Target Information

TNFR1 (Tumor necrosis factor receptor 1) belongs to the tumor necrosis factor superfamily, and is one of the major TNF-alpha receptors. TNFR1 plays an important role in mediating, in cytokine mediated signaling, positive regulation of the NF-Kb pathway, positive regulation of angiogenesis, and negative regulation of gene expression. The extracellular domain of TNFR1 is also released into the circulatory system as soluble TNFR1 (sTNFR1). In humans, the TNFR1 gene is located on chromosome 12. Anti-apoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with TNFR1, and thus play regulatory roles in the signal transduction mediated by the receptor. Germline mutations of the extracellular domains of TNFR1 were found to be associated with the autosomal dominant periodic fever syndrome, and the impaired receptor clearance is thought to be a mechanism of the disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.