Thermo Fisher Scientific TNFR1 Monoclonal Antibody (16805)
인간 TNFR1에 특이적인 Mouse IgG1 단클론 항체로 Western blot, ELISA, 중화 실험에 사용 가능. E. coli 유래 재조합 TNFRSF1A 항원으로 제조되었으며, 단백질 A/G 정제 및 무보존제 PBS 버퍼에 동결건조 형태로 제공. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| ELISA (Detection) | 0.1–0.4 µg/mL |
| ELISA (Capture) | 2–8 µg/mL |
| Neutralization (Neu) | 1–6 µg/mL |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 16805 |
| Immunogen | E. coli-derived recombinant human TNF RI/TNFRSF1A extracellular domain |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.5 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2609265 |
Product Specific Information
- In sandwich ELISAs, no significant cross-reactivity or interference with recombinant human or porcine TGF-alpha, TGF-beta isoforms, TNF-beta, or TNF RII is observed.
- Reconstitute at 0.5 mg/mL in sterile PBS.
- Endotoxin level: <0.10 EU per 1 µg of antibody (LAL method).
Target Information
TNFR1 (Tumor necrosis factor receptor 1) is a member of the TNF superfamily and a major receptor for TNF-alpha. It mediates cytokine signaling, regulates NF-κB pathway activation, angiogenesis, and gene expression.
The extracellular domain can be released as soluble TNFR1 (sTNFR1).
The TNFR1 gene is located on chromosome 12.
TNFR1 interacts with adaptor proteins TRADD, TRAF2, and BAG4/SODD, which modulate signal transduction.
Mutations in the TNFR1 extracellular domain are associated with autosomal dominant periodic fever syndrome due to impaired receptor clearance.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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