Thermo Fisher Scientific NEDD8 Polyclonal Antibody
NEDD8 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB, IHC, ICC, IP 등 다양한 응용에 적합합니다. 인간, 마우스, 설치류 등 여러 종과 반응하며, 높은 특이성과 비교반응성을 제공합니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 | - |
| Immunocytochemistry (ICC/IF) | 1:100 | - |
| Immunoprecipitation (IP) | 1:25 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids at the amino-terminus of human NEDD8 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 162 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10984681 |
Product Specific Information
- It is not recommended to aliquot this antibody.
- This antibody is not cross-reactive with other ubiquitin family members, including ubiquitin, SUMO1, SUMO2, SUMO3, and ISG15.
Target Information
NEDD8 is a ubiquitin-like protein that plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, promoting polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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