Thermo Fisher Scientific Phospho-c-Raf (Ser259) Recombinant Rabbit Monoclonal Antibody (ARC1557)
Phospho-c-Raf (Ser259) 단백질을 특이적으로 인식하는 재조합 토끼 단클론 항체로, Western blot 및 ELISA에 적합합니다. HEK293 세포 발현 시스템에서 생산되며, 고순도 친화 크로마토그래피로 정제되었습니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ARC1557 |
| Immunogen | A synthetic phosphorylated peptide around S259 of human RAF1 (P04049) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.25 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2849760 |
Product Specific Information
- Immunogen sequence: STSTP
Target Information
Raf-1 (c-Raf) is a MAP kinase kinase kinase (MAP3K) functioning downstream of the Ras family of membrane-associated GTPases. Upon activation, Raf-1 phosphorylates and activates MEK1 and MEK2, which in turn activate ERK1 and ERK2. Activated ERKs regulate gene expression involved in cell division, apoptosis, differentiation, and migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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