Thermo Fisher Scientific CLEC11A Polyclonal Antibody, Biotin, PeproTech
인간 CLEC11A(SCGF-beta)를 인식하는 염소 유래 Biotin 결합 폴리클로날 항체. Western blot 및 ELISA에 적합하며, 항원 친화 크로마토그래피로 정제됨. 동결건조 형태로 제공되며, -20°C에서 보관. 연구용으로만 사용 가능.
- 카탈로그번호
- 500-P99GBT-500UG
- 판매단위
- pk
카탈로그
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.25–1.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived, 25.0 kDa Recombinant Human SCGF-beta |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
Product Specific Information
Amino Acid Sequence of Recombinant Protein:
MGHGARGAER EWEGGWGGAQ EEEREREALM LKHLQEALGL PAGRGDENPA GTVEGKEDWE MEEDQGEEEE EEATPTPSSG PSPSPTPEDI VTYILGRLAG LDAGLHQLHV RLHALDTRVV ELTQGLRQLR NAAGDTRDAV QALQEAQGRA EREHGRLEGC LKGLRLGHKC FLLSRDFEAQ PSASPHPLSP DQPNGGTLEN CVAQASDDGS WWDHDCQRRL YYVCEFPF.
Preparation:
Produced from sera of goats immunized with highly pure Recombinant Human SCGF-beta. Anti-Human SCGF-beta-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA:
To detect hSCGF-beta by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human SCGF-beta (500-P99G) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant hSCGF-beta.
Western Blot:
To detect hSCGF-beta by Western blot, use at 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant hSCGF-beta is 1.5–3.0 ng/lane under reducing or non-reducing conditions.
Packaging:
500-P99GBT-1MG is provided as 2 × 500 µg.
Target Information
This gene encodes a member of the C-type lectin superfamily. The encoded protein is a secreted sulfated glycoprotein functioning as a growth factor for primitive hematopoietic progenitor cells. An alternative splice variant has been described but its biological nature has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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