Thermo Fisher Scientific CLEC11A Polyclonal Antibody, Biotin, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

-

ELISA (ELISA)

0.25-1.0 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Goat

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived, 25.0 kDa Recombinant Human SCGF-beta if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CLEC11A,uniProtId:Q9Y240-1,ncbiNodeId:9606,antigenRange:22-323,antigenLength:323,antigenImageFileName:500-P99GBT-25UG_CLEC11A_Q9Y240-1_Goat.svg,antigenImageFileNamePDP:500-P99GBT-25UG_CLEC11A_Q9Y240-1_Goat_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Biotin Biotin Biotin

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2930903

Product Specific Information

AA Sequence of recombinant protein: MGHGARGAER EWEGGWGGAQ EEEREREALM LKHLQEALGL PAGRGDENPA GTVEGKEDWE MEEDQGEEEE EEATPTPSSG PSPSPTPEDI VTYILGRLAG LDAGLHQLHV RLHALDTRVV ELTQGLRQLR NAAGDTRDAV QALQEAQGRA EREHGRLEGC LKGLRLGHKC FLLSRDFEAQ PSASPHPLSP DQPNGGTLEN CVAQASDDGS WWDHDCQRRL YYVCEFPF.

Preparation: Produced from sera of goats immunized with highly pure Recombinant Human SCGF-beta. Anti-Human SCGF-beta-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect hSCGF-beta by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human SCGF-beta (500-P99G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hSCGF-beta.

Western Blot: To detect hSCGF-beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hSCGF-beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P99GBT-1MG will be provided as 2 x 500 µg

Target Information

This gene encodes a member of the C-type lectin superfamily. The encoded protein is a secreted sulfated glycoprotein and functions as a growth factor for primitive hematopoietic progenitor cells. An alternative splice variant has been described but its biological nature has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.