Takara SARS-CoV-2 pseudovirus packaging systems - B.1.351

Lenti-X SARS-CoV-2 Packaging Single Shots enable streamlined, high-titer production of lentiviral particles pseudotyped with SARS-CoV-2 spike protein; simply add water containing your transfer vector of choice to a tube of single shots and then apply the reconstituted transfection mix to packaging cells in a 10-cm dish. For your convenience, Lenti-X SARS-CoV-2 packaging mixes are sold separately or bundled with ZsGreen1, luciferase transfer vectors, and negative control packaging reagents.

Lenti-X SARS-CoV-2 Packaging Single Shots enable streamlined, high-titer production of lentiviral particles pseudotyped with SARS-CoV-2 spike protein; simply add water containing your transfer vector of choice to a tube of single shots and then apply the reconstituted transfection mix to packaging cells in a 10-cm dish. For your convenience, Lenti-X SARS-CoV-2 packaging mixes are sold separately or bundled with ZsGreen1, luciferase transfer vectors, and negative control packaging reagents.

Separate Lenti-X packaging mixes are available for production of pseudovirus bearing full-length or truncated forms of the spike protein from the SARS-CoV-2 variant B.1.351 (originally identified in South Africa). The truncation involves removal of 19 amino acids encoding an ER retention signal on the protein C-terminus, and is associated with increased abundance of spike protein on the resulting particles, and increased particle infectivity (Johnson et al_._ 2020).

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