Thermo Fisher Scientific Protein G Polyclonal Antibody
Protein G에 특이적인 Rabbit Polyclonal Antibody로 다양한 면역실험(WB, IHC, ELISA, IP)에 적용 가능. 고순도 이온교환 크로마토그래피로 정제되어 높은 특이성과 재현성 제공. 단기간 4°C, 장기간 -20°C 보관 권장. 연구용으로만 사용.
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Applications
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | |
| Immunohistochemistry (IHC) | Assay-dependent | |
| ELISA | 1:20,000–1:100,000 | |
| Immunoprecipitation (IP) | 1:2,000–1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bacteria |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Protein G (Streptococcus species) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | 0.02 M potassium phosphate, pH 7.2, with 0.15 M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Protein G. No cross reactivity occurs against Protein A.
Target Information
Protein G is an immunoglobulin-binding protein expressed in group C and G Streptococcal bacteria, similar to Protein A but with differing binding specificities. It is a cell surface protein used for antibody purification through its binding to the Fab and Fc regions. The native molecule also binds albumin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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