Thermo Fisher Scientific MTOR Monoclonal Antibody (OTI5B8), TrueMAB
Human mTOR 단백질(aa1766-2144)을 인식하는 Mouse IgG2a 단클론 항체. Western blot 및 IHC(P) 검증 완료. Lyophilized 형태로 제공되며, PBS buffer(8% trehalose) 보존. 연구용으로 세포 성장 및 암 관련 신호전달 연구에 적합.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:150
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5B8 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1766–2144 of human MTOR produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
FRAP1 (mTOR) is a serine/threonine kinase essential for cellular growth and proliferation. Dysregulation of the mTOR/PI3K/AKT pathway is linked to various cancers. FRAP1 is the target of rapamycin and its analogs, used as immunosuppressants and cancer therapeutics. Mutations in this gene cause Smith-Kingsmore syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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