Thermo Fisher Scientific c-MAF Monoclonal Antibody (sym0F1), eFluor 450, eBioscience
Human 및 Mouse c-Maf 단백질을 인식하는 sym0F1 단클론 항체로, eFluor 450 형광체가 결합되어 Flow Cytometry에 최적화됨. IL-4, IL-21, IL-23R, CXCR5 발현 연구에 활용 가능. 405/445 nm Excitation/Emission 특성으로 Violet Laser 검출에 적합.
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Thermo Fisher Scientific c-MAF Monoclonal Antibody (sym0F1), eFluor 450, eBioscience
Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 5 µL (0.125 µg)/test
- Publications: View 2 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG2b, kappa |
| Recommended Isotype Control | Mouse IgG2b kappa Isotype Control (eBMG2b), eFluor™ 450, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | sym0F1 |
| Immunogen | Full-length E. coli-produced c-Maf |
| Conjugate | eFluor™ 450 |
| Excitation/Emission Max | 405/445 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2762608 |
Additional Formats
- Alexa Fluor 488
- Brilliant Violet 421
- Brilliant Violet 480
- eFluor 660
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
The sym0F1 monoclonal antibody reacts with human and mouse c-Maf, a 42 kDa basic leucine zipper transcription factor involved in neural, ocular, and hematopoietic systems. In hematopoietic cells, c-Maf is crucial for IL-4 expression in Th2 and plays a role in Th17 and Tfh cell differentiation and function. It drives IL-21 expression and promotes IL-23R and CXCR5 expression.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Intracellular staining of Th17-polarized mouse splenocytes using the Foxp3/Transcription Factor Buffer Set and protocol
- Use 5 µL (0.125 µg) per test (100 µL final volume)
- Applicable for 10⁵–10⁸ cells/test
- Refer to BestProtocols® – Staining Intracellular Antigens for Flow Cytometry
Fluorochrome Information:
eFluor 450 is an alternative to Pacific Blue.
- Excitation: 405 nm
- Emission: 445 nm
- Laser: Violet Laser
Ensure your instrument can detect this fluorochrome.
Target Information
c-maf is the cellular counterpart of oncogenic v-maf belonging to the basic region leucine zipper transcription factor family. It interacts with LRPICD and exists in two mRNA forms (long and short). c-maf regulates IL-4, crystalline genes, insulin, p53, and L7 through MARE binding. It controls Th2 differentiation and IL-10 expression in macrophages. Chromosomal aberrations involving maf are observed in multiple myeloma, where it is expressed in myeloma cell lines and resting monocytes/macrophages.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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