Thermo Fisher Scientific c-MAF Monoclonal Antibody (sym0F1), eFluor 450, eBioscience

Thermo Fisher Scientific c-MAF Monoclonal Antibody (sym0F1), eFluor 450, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.125 µg)/test
• Publications: View 2 publications

Product Specifications

Additional Formats

• Alexa Fluor 488
• Brilliant Violet 421
• Brilliant Violet 480
• eFluor 660
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The sym0F1 monoclonal antibody reacts with human and mouse c-Maf, a 42 kDa basic leucine zipper transcription factor involved in neural, ocular, and hematopoietic systems. In hematopoietic cells, c-Maf is crucial for IL-4 expression in Th2 and plays a role in Th17 and Tfh cell differentiation and function. It drives IL-21 expression and promotes IL-23R and CXCR5 expression.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Intracellular staining of Th17-polarized mouse splenocytes using the Foxp3/Transcription Factor Buffer Set and protocol
  • Use 5 µL (0.125 µg) per test (100 µL final volume)
  • Applicable for 10⁵–10⁸ cells/test
  • Refer to BestProtocols® – Staining Intracellular Antigens for Flow Cytometry

Fluorochrome Information:
eFluor 450 is an alternative to Pacific Blue.

• Excitation: 405 nm
• Emission: 445 nm
• Laser: Violet Laser

Ensure your instrument can detect this fluorochrome.

Target Information

c-maf is the cellular counterpart of oncogenic v-maf belonging to the basic region leucine zipper transcription factor family. It interacts with LRPICD and exists in two mRNA forms (long and short). c-maf regulates IL-4, crystalline genes, insulin, p53, and L7 through MARE binding. It controls Th2 differentiation and IL-10 expression in macrophages. Chromosomal aberrations involving maf are observed in multiple myeloma, where it is expressed in myeloma cell lines and resting monocytes/macrophages.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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