Thermo Fisher Scientific c-MAF Monoclonal Antibody (sym0F1), eFluor 450, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Not Applicable

Host/Isotype

Mouse / IgG2b, kappa

Recommended Isotype Control

Mouse IgG2b kappa Isotype Control (eBMG2b), eFluor™ 450, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

sym0F1

Immunogen

Full-length E. coli-produced c-Maf if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:c-MAF,uniProtId:O75444-2,ncbiNodeId:9606,antigenRange:1-373,antigenLength:373,antigenImageFileName:48-9855-42_c-MAF_O75444-2_House_mouse.svg,antigenImageFileNamePDP:48-9855-42_c-MAF_O75444-2_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

eFluor™ 450 eFluor™ 450 eFluor™ 450

View additional formats

• Alexa Fluor 488
• Brilliant Violet 421
• Brilliant Violet 480
• eFluor 660
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Excitation/Emission Max

405/445 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2762608

Product Specific Information

Description: The sym0F1 monoclonal antibody reacts with human and mouse c-Maf, a 42 kDa basic leucine zipper transcription factor shown to be involved in the neural, ocular and hematopoietic systems. In hematopoietic cells, it was first shown to be crucial for IL-4 expression in Th2 and was the first transcription factor believed to be Th subset-specific. More recent evidence shows that specific phospho-tyrosine residues lead to upregulation of IL-4. c-Maf has also been shown to be important to differentiation and function in both Th17 and Tfh cells. It drives expression of IL-21 in both cell types, while promoting expression of IL-23R in Th17 and CXCR5 in Tfh as well.

Applications Reported: This sym0F1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This sym0F1 antibody has been pre-diluted and tested by intracellular staining followed by flow cytometric analysis of Th17-polarized mouse splenocytes using the Foxp3/Transcription Factor Buffer Set and protocol. Please see BestProtocols® Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor 450 is a alternative to Pacific Blue.eFluor 450 emits at 445 nm and is excited with the violet laser line (405 nm). Please make sure that your instrument is capable of detecting this fluorochrome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser

Target Information

c-maf is the cellular counterpart of oncogenic v-maf that belongs to the family of basic region leucine zipper domain transcription factors. The leucine-zipper domain is involved in the interaction with LRPICD. There are two forms of human c-maf mRNA, c-maf-long and c-maf-short. It is identified in the genome of the acute transforming avian retrovirus AS42. c-maf targets are IL-4 in Th2 cells, the crystalline genes in lens fiber cells, insulin gene in islet, p53 and L7 where it exerts its transcriptional role through binding to a Maf recognition element (MARE). It regulates Th2 differentiation and lineage-specific hematopoiesis. c-maf is a transcription factor for IL-10 gene expression in LPS-activated macrophages. Chromosomal aberration involving maf is found in some forms of multiple myeloma. It is expressed in myeloma cell lines and resting monocytes/macrophages.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.