Thermo Fisher Scientific Epiregulin Polyclonal Antibody
Epiregulin 단백질을 인식하는 Goat Polyclonal Antibody로, Western blot, IHC, ELISA, Neutralization에 사용 가능. 인간 시료 반응성. 항원 친화 크로마토그래피로 정제되었으며, PBS + 5% 트레할로스 완충액에 동결건조 형태로 제공.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1 µg/mL | - |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5–15 µg/mL | - |
| ELISA | Assay-Dependent | - |
| Neutralization (Neu) | 0.3–1.5 µg/mL | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E. coli-derived recombinant human Epiregulin (Val63–Leu108) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2610406 |
Product Specific Information
- In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant mouse Epiregulin is observed.
- Reconstitute at 0.2 mg/mL in sterile PBS.
- Endotoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
Epiregulin is an EGF-related growth factor that binds specifically to EGFR (ErbB1) and ErbB4, but not to ErbB2 or ErbB3. It is mainly expressed in the placenta and peripheral blood leukocytes, as well as in certain carcinomas of the bladder, lung, kidney, and colon. Epiregulin stimulates the proliferation of keratinocytes, hepatocytes, fibroblasts, and vascular smooth muscle cells, while inhibiting the growth of several tumor-derived epithelial cell lines. Human Epiregulin is initially synthesized as a glycosylated 19.0 kDa transmembrane precursor protein, which is processed by proteolytic cleavage to produce a 6.0 kDa mature secreted sequence.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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