
Thermo Fisher Scientific Goat anti-Feline IgG (H+L) Secondary Antibody, HRP
고양이 IgG(H+L)에 특이적인 HRP 결합 2차 항체로, Western blot, IHC, ELISA 등 다양한 면역실험에 사용 가능. 염색체 친화성 정제된 고순도 제품으로 높은 민감도와 특이성을 제공. 연구용으로만 사용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Cat |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobulins Heavy and Light chains |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.1% ProClin 150 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2535534 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Additional Formats
Product Specific Information
Each lot of antibody is tested for sensitivity using ELISA and for specificity using immunoelectrophoresis (IEP).
Rehydrate with 0.55 mL of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove particulates. Prepare fresh working dilution daily.
Store lyophilized material at 2–8°C. For long-term storage after reconstitution, dilute with 50% glycerol and store at -20°C as a liquid.
Based on immunoelectrophoresis, no reactivity is observed to non-immunoglobulin cat serum immunoglobulins.
Target Information
Anti-Feline secondary antibodies are affinity-purified antibodies with well-characterized specificity for feline immunoglobulins. They are useful in detection, sorting, or purification of feline IgG targets.
Secondary antibodies offer versatility, enabling use with multiple detection systems (HRP, AP, fluorescence) and provide enhanced sensitivity via signal amplification.
Typically, secondary antibodies are generated by immunizing host animals with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to ensure high specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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