Thermo Fisher Scientific Goat anti-Bovine IgG (H+L) Secondary Antibody, AP
염소 유래 항-소 IgG(H+L) 2차 항체로 AP 결합 형태. Western blot, IHC, ELISA 등 다양한 응용 가능. 높은 특이성과 감도를 제공하며, 친화 크로마토그래피로 정제됨. 연구용으로만 사용.
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Thermo Fisher Scientific Goat anti-Bovine IgG (H+L) Secondary Antibody, AP
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Bovine IgG (H+L) |
| Conjugate | AP |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 30 mM triethanolamine, 1% BSA, 5 mM MgCl₂, 0.1 mM ZnCl₂ |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2535531 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Additional Formats Available
- Agarose
- FITC
- HRP
- Texas Red
- TRITC
- Custom conjugation available upon request
Product Specific Information
The sensitivity of each lot of antibody is confirmed using ELISA.
The specificity of each lot of antibody is confirmed by immunoelectrophoresis (IEP).
Target Information
Anti-Bovine secondary antibodies are affinity-purified antibodies with well-characterized specificity for bovine (cow, ox) immunoglobulins.
They are useful in detection, sorting, or purification of the specified target.
Secondary antibodies offer increased versatility, enabling use with various detection systems (e.g., HRP, AP, fluorescence).
They can provide greater sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Typically, secondary antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to produce highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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