
Thermo Fisher Scientific Goat anti-Rabbit IgG Fc Cross-Adsorbed Secondary Antibody, Biotin
염소 유래의 항-토끼 IgG Fc 교차흡착 2차 항체로 Biotin 결합형입니다. Western blot, ICC/IF, Flow cytometry, ELISA 등 다양한 면역분석에 적합합니다. 높은 특이성과 낮은 비특이적 결합으로 신뢰성 높은 신호 검출이 가능합니다.
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Applications 및 희석 배수
| 응용 분야 | 권장 희석 배수 |
|---|---|
| Western Blot (WB) | 1:10,000–1:50,000 |
| Immunocytochemistry (ICC/IF) | 1:500–1:20,000 |
| Flow Cytometry (Flow) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
제품 사양 (Product Specifications)
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rabbit |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobulin Fc region |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2534799 |
| Target | IgG Fc |
| Cross Adsorption | Against bovine, horse, and human serum proteins |
| Antibody Form | Whole Antibody |
추가 포맷 (Available Conjugates)
Target Information
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins. They are useful for the detection, sorting, or purification of rabbit targets.
Secondary antibodies provide versatility by enabling various detection systems (e.g., HRP, AP, fluorescence) and can enhance sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Typically, secondary antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species and may be further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to achieve high specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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