
Thermo Fisher Scientific Rabbit anti-Chicken IgY (H+L) Secondary Antibody, HRP
Rabbit에서 유래한 Polyclonal HRP-conjugated anti-Chicken IgY(H+L) 2차 항체로, Western blot, IHC, ELISA에 사용 가능. 높은 특이성과 감도로 검증되었으며, 친화 크로마토그래피로 정제됨. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Chicken IgY (H+L) |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.1% ProClin 150 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2534801 |
| Target | IgY |
| Antibody Form | Whole Antibody |
Additional Formats
- [AP]
- [Biotin]
- [FITC]
- [Rhodamine]
- [TRITC]
- [Request custom conjugation]
Product Specific Information
Each antibody lot is validated for sensitivity using ELISA and for specificity by immunoelectrophoresis (IEP).
Rehydrate with 1.1 mL deionized water and allow to stand for 30 minutes at room temperature to dissolve. Centrifuge to remove particulates. Prepare fresh working dilution daily.
Store lyophilized material at 2–8 °C. For long-term storage after reconstitution, dilute with 50% glycerol and store at -20 °C as a liquid.
Based on immunoelectrophoresis, no reactivity is observed to non-immunoglobulin chicken serum proteins.
Target Information
Anti-Chicken secondary antibodies are affinity-purified and provide specific detection of chicken immunoglobulins. They are useful for detection, sorting, or purification of target proteins.
Secondary antibodies enhance assay versatility and sensitivity by enabling various detection systems (e.g., HRP, AP, fluorescence) and signal amplification.
These antibodies are typically generated by immunizing host animals with pooled immunoglobulins from the target species and further purified or modified for high specificity and performance.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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