Thermo Fisher Scientific Cytochrome C (Mitochondrial Marker) Monoclonal Antibody (SPM389)
미토콘드리아 마커로 사용되는 Cytochrome C 단백질을 인식하는 단클론 항체입니다. Western blot 및 면역조직화학(IHC)에 적합하며, 다양한 종에 반응합니다. 단백질 A/G 정제 및 PBS 완충액에 보관되며, 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Amphibian, Avian, Dog, Fruit fly, Horse, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SPM389 |
| Immunogen | Synthetic peptides corresponding to amino acid 1–80, 81–104 and 66–104 of pigeon cytochrome c |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage conditions | 4°C, do not freeze |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Target Information
Cytochrome C is an electron-transporting protein located in the mitochondrial intermembrane space, playing a crucial role in oxidative phosphorylation and ATP production. Upon apoptotic stimulation, cytochrome C is released into the cytosol, where it binds apoptotic protease activating factor, leading to activation of procaspase 9 and subsequently caspase 3, a key effector of apoptosis.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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