Thermo Fisher Scientific GORAB Monoclonal Antibody (OTI2E6), TrueMAB
GORAB 단백질을 표적으로 하는 Mouse IgG1 단클론 항체로, Western blot, ICC/IF, Flow cytometry에 적합. 인간 시료 반응성, 고순도 친화 크로마토그래피 정제. 동결건조 형태로 제공되며, 재용해 및 결합 실험에 최적화됨.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2E6 |
| Immunogen | Human recombinant protein fragment (aa 35–394 of human GORAB) produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
TCEB1, also known as elongin C, is a subunit of the transcription factor B (SIII) complex composed of elongins A/A2, B, and C. This complex activates elongation by RNA polymerase II by suppressing transient pausing during transcription.
Elongin A acts as the transcriptionally active component, while elongins B and C serve regulatory roles. Elongin A2 is testis-specific and forms a stable complex with elongins B and C.
The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, inhibiting transcription elongation. Western blot analyses using two distinct antibodies targeting unique regions of this protein confirm consistent molecular weight results.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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