Thermo Fisher Scientific GORAB Monoclonal Antibody (OTI1G2), TrueMAB
GORAB 단백질을 인식하는 Mouse Monoclonal 항체로 Western Blot 및 IHC(P)에서 검증됨. Human 시료에 반응하며, carrier-free 형태로 제공되어 conjugation 실험에 적합. 동결건조 상태로 제공되며, 재구성 후 1 mg/mL 농도로 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1G2 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 35–394 of human GORAB produced in E. coli. |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of about 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
TCEB1, also known as protein elongin C, is a subunit of the transcription factor B (SIII) complex, which includes elongins A/A2, B, and C. This complex activates elongation by RNA polymerase II by suppressing transient pausing during transcription.
Elongin A acts as the active component, while elongins B and C serve as regulatory subunits. Elongin A2 is testis-specific and forms a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, inhibiting transcription elongation. Western blot analysis using two distinct antibodies against unique regions of this protein confirmed consistent molecular weight results.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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