Thermo Fisher Scientific GORAB Monoclonal Antibody (OTI1E3), TrueMAB
GORAB 단백질을 인식하는 Mouse monoclonal 항체로 Western blot 및 IHC(P)에서 검증됨. Human, Mouse, Rat, Dog 반응성. Lyophilized 형태로 제공되며, 재구성 후 1 mg/mL 농도. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1E3 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 35–394 of human GORAB produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | –20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).
Target Information
TCEB1, also known as protein elongin C, is a subunit of the transcription factor B (SIII) complex, which includes elongins A/A2, B, and C. This complex enhances RNA polymerase II elongation by reducing transient pausing during transcription.
Elongin A acts as the active transcriptional component, while elongins B and C serve regulatory roles. Elongin A2 is testis-specific and forms a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein interacts with elongins B and C, inhibiting transcription elongation.
Western blot analysis using two different antibodies (P100962-T200 and P100963_P050 / P100963_P100) targeting unique regions of this protein confirmed consistent molecular weight results.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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