Thermo Fisher Scientific F(ab)-Goat anti-Mouse IgG1 Fc Secondary Antibody, Alexa Fluor 594

Applications

Tested Dilution

Immunohistochemistry (Paraffin) (IHC (P))

1 µg/mL

Immunohistochemistry (PFA fixed) (IHC (PFA))

1 µg/mL

Immunocytochemistry (ICC/IF)

0.5-2 µg/mL

Flow Cytometry (Flow)

0.5-2 µg/mL

Peptide Array (Array)

Not Cited

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Goat / IgG, F(ab)

Class

Polyclonal

Type

Secondary Antibody

Immunogen

Mouse IgG1 Fc

Conjugate

Alexa Fluor™ 594 Alexa Fluor™ 594 Alexa Fluor™ 594

View additional formats

• [Alexa Fluor 405 ](/antibody/secondary/product-name/Mouse IgG1 Fc/conjugates/Alexa Fluor 405/host/F(ab)-Goat/)
• [Alexa Fluor 488 ](/antibody/secondary/product-name/Mouse IgG1 Fc/conjugates/Alexa Fluor 488/host/F(ab)-Goat/)
• [Alexa Fluor 555 ](/antibody/secondary/product-name/Mouse IgG1 Fc/conjugates/Alexa Fluor 555/host/F(ab)-Goat/)
• [Alexa Fluor 568 ](/antibody/secondary/product-name/Mouse IgG1 Fc/conjugates/Alexa Fluor 568/host/F(ab)-Goat/)
• [Alexa Fluor 647 ](/antibody/secondary/product-name/Mouse IgG1 Fc/conjugates/Alexa Fluor 647/host/F(ab)-Goat/)
• [Alexa Fluor 700 ](/antibody/secondary/product-name/Mouse IgG1 Fc/conjugates/Alexa Fluor 700/host/F(ab)-Goat/)
• Request custom conjugation

Excitation/Emission Max

590/618 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Size-exclusion chromatography

Storage buffer

PBS, pH 7.5

Contains

5mM sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient

Target

IgG Fc

Antibody Form

F(ab) Fragment

Product Specific Information

This F(ab)-Goat anti-Mouse IgG1 Fc specific antibody product is a goat polyclonal antibody F(ab) fragment that is specific to the Fc region of mouse IgG1 isotype. The product has minimal to no cross-reactivity towards mouse IgG2a, mouse IgG2b, mouse IgG3, polyclonal rat, rabbit, guinea pig, bovine, hamster, horse, sheep, human, monkey, donkey IgG, and chicken IgY as tested by direct ELISA. May show mild cross-reactivity with rat IgG2a. Cross-reactivity towards other immunoglobulins has not been tested. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

F(ab) antibody fragment offer several benefits over whole IgGs, due to their smaller size (~50 kD vs. ~150 kD) and the absence of the Fc region. The smaller size is expected to allow better penetration into tissue sections and improved staining in applications such as IHC. The lack of Fc region results in reduced nonspecific background staining when staining cells/ tissues with Fc receptors (such as immune cells) and other Fc interaction-related interference. Fc specific F(ab) fragments provide a range of multiplexing options beyond what is possible with whole IgGs for applications such as immunocytochemistry, immunohistochemistry, flow cytometry, and western blotting.

Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.

Target Information

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.